4.0 Determination of Sample Volume Required for Analyses
4.1 10 mL Preliminary Investigation
The 10 mL preliminary investigation is usually performed by the soft algae analysts in order to
determine the appropriate volume of sample required for both soft algae and diatom analyses.
4.1.1 Apparatus
4.1.1.1 lnverted microscope with an objective system for magnification up to 150x (Leitz
Diavert or another equal quality inverted microscope).
4.1.1.2 Tubular plankton chamber or combined plate chamber 10 cc.
4.1.1.3 Cover plate for plankton chamber, 33 mm dia., 2 mm thick.
4.1.1.4 Base plate for plankton chamber, 27.5 mm dia., 0.2 mm thick.
4.1.1.5 10 mL automacropipette.