The leghaemoglobin (Lb) content in fresh nodules collected from the roots of both inoculated and un-inoculated plants was measured at 50 DAS (Sadasivam and Manikam, 1992) while total N and P content in roots and shoots was assayed at 80 DAS by micro-Kjeldahl (Iswaran and Marwah, 1980) and Jackson (1967) method, respectively