The RNA viral amplification was developed using the QuantiTect™ SYBR® Green RT-PCR kit (Qiagen S.A., Hilden, Germany). Real-time PCRs were run on the thermocyclers Rotor Gene Q (Qiagen S.A., Hilden, Germany).
The RNA viral amplification was developed using theQuantiTect™ SYBR® Green RT-PCR kit (Qiagen S.A., Hilden,Germany). Real-time PCRs were run on the thermocyclers RotorGene Q (Qiagen S.A., Hilden, Germany).