Consumption of glucose and acetate levels in defined mediawas detected in a Shimadzu UFLC with a Shim-pack SCR-101Hcolumn with a mobile phase of H2SO4running at 0.6 mL min−1.One milliliter of fermentation samples were collected hourly andcentrifuged at 20,000 × g for 2 min. Standards were prepared withserial dilutions of known concentration of glucose and acetate.Detection was with refractive index (RI) or UV 210 nm with theacetatepeak having a retention time of about 9 min and the glucose,14 min.