The quantitative study revealed that formulation OD3 considerable increase in
number of hair follicle in anagen phase of hair growth cycle, when compare to standard and
control. In standard group percentage of population of anagen follicle was 67%, where as in
formulation OD3 it was 83% and OD2 81%. Similarly the formulation OD1 and OD2 were
shown time of initiation of hair growth late when compared with standard and control. It
also observed that the time taken for complete hair growth the late initiation and completion
of hair growth was 25d in OD1 and 23d in OD2 indicating late initiation and completion of
hair growth. The result shows that formulation OD3 have contributed in most significant
hair growth activity. Similarly, the way of method of preparation of OD3 (7.5%
concentration of all drugs) boiling in pouch method showed maximum extraction of active
principles responsible for hair growth. The hair growth studies finally prove that formulation
OD3 have significant increase in hair growth activity when compared to the standard. It
holds the promise of potent herbal alternative for minoxidil. Also suggest excellent results of
hair growth in formula prepared by cloth pouch boiling method.