We isolated and identified three protease-producing bacteria that had inhabited the region
around the Korean Arctic Research Station Dasan located at Ny-Alesund, Svalbard, Norway (79o
N, 12o
E).
Biofilms were collected from the surface of a floating pier and from dead brown algae in a tide pool near
the seashore.The biofilm samples were transported to the Korea Polar Research Institute (KOPRI) under
frozen conditions, diluted in sterilized seawater, and cultured on Zobell agar plates with 1% skim milk at
10o
C Three clear zone forming colonies were selected as protease-producing bacteria