Similar results were obtained at 25 C (Figures 7M and 7N).
We found that flies lacking azot (azot/) had a shortened
lifespan with a median survival of 25 days, which represented
a 24% decrease when compared to WT flies (azot+/+), and
a maximum lifespan of 40 days, 31% less than WT flies
(azot+/+). On the contrary, flies with three functional copies of
the gene (azot+/+; azot+) or flies where azot is replaced by hid
(azot{KO; hid} homozygous flies) showed an increase in median
survival of 54% and 63% and maximum lifespan of 12% and
24%, respectively