Porcine intestines harvested, cleaned, boxed and ready for commerce using industry
standard procedures were tested for the presence of general aerobic bacteria and generic
E.coli. Half of the samples collected were subjected to aqueous ozone at a concentration
of about 2.3 ppm and the other half of the samples were left untreated. All samples were
analyzed and it was found that the treated samples had an average 1.9 log reduction of
general aerobic bacteria and a 1.7 log reduction of E.coli when compared to the untreated
samples. In general, any antimicrobial that can achieve greater than a 1 log pathogen
reduction is considered to be significantly efficacious.
Methods:
10 separate hog intestines (bung/large intestine) were removed from their box where they
had been placed ready for commerce (all processes complete). An approximate 5 cm
section was cut off from each of the 10 samples and then incisions were made to open
them. 5 of these sample sections were placed into individual Whirl-Pak sample bags and
placed aside. The other 5 sections were laid flat upon a sanitary surface in the Hot Box
and both sides were sprayed with aqueous ozone from our application system. The
concentration of ozone was tested to be approximately 2.3 ppm (based on color
comparisons to standards). Each of the treated samples was then placed into individual
Whirl-Pak sample bags. 25 ml of sterile buffer was added to each of the 10 sample bags
in preparation for plating. After stomaching all samples were plated onto individual 3M
Petrifilms for APC and for generic E.coli, then incubated at 36 degrees C for 24 hours.
All resulting plates were then counted.