The minimum inhibitory concentration (MIC) of the oils was determined using microtitre plates for a serial two-fold broth dilution (CLSI, 2009) with the following modifications.Using essential oil,oil emulsions were prepared by vortexing a measured combination of oil and the appropriate broth with 0.15% w/w agar
(Mann andMarkham,1998). Using extracts,dried material was weighed and dissolved into a 1:1 mixture of acetone and distilled water , then mixed into the 0.15% w/w agar in Tryptone Soya Broth at 3%v/v as the starting concentration,with1.5% acetone as a control.Undried extracts,such as H2O extracts,were mixed at 10% starting concentration.