organism. The specific role of T and other hormones and factors
with putative action in the ovaries has been investigated in iso-
lated ovarian cells. Quail pooled granulosa cells from F1 to F3 fol-
licles were cultured for 66 h with 1 lg/ml of T or E2 and, whilst P4
production after E2 treatment was 2.5 times that of the control, the
response to T administration was 14 times larger.