2.5. Visual inspection of emulsion stability and accelerated stability
test by centrifugation
Immediately after preparation, 6 ml emulsion was transferred
into a plastic centrifuge tube and centrifuged at 4000g for 5 min
to accelerate the phase separation. A layer of floated oil, a concentrated
layer of droplets (designated as creamed emulsions) and a
clear bottom phase were usually observed and their volumes were
calculated from their thickness, which were measured with a digital
caliper. These volumes were used to calculate the cream fraction
(volume of creamed emulsion/total volume of emulsion), the
oil fraction in cream (volume of dodecane/volume of creamed
emulsion) and the usage level of chitin (g/g oil) (Jia et al., 2015).
All experiments were repeated in triplicate and data are represented
as mean ± standard derivation.