The lowest RMSECV of the all-trans lycopene content was achieved by considering two PLS factors and the first derivative + MSC pre-treatment. The correlation between the NIR measurement and the all-trans lycopene content of tomatoes was adequately high (R2CV= 0.75; RMSECV = 6.88 μg g-1). Baranska et al. (2006) (R2CV= 0.927, RMSECV = 8.64μg g-1) and Tianhua et al. (2013) performed a similar experiment but the size of the sample set used in that report was much smaller (28 samples were used) and the SECV value was more than ten times higher (91.2 mg 100 g-1) than in our study. Pedro and Ferreira (2005) applied NIR spectra (1 000-2 500 nm) and multivariate calibration to evaluate lycopene and β-carotene contents of tomato. They found better results using a spectra-splitting approach, using MSC than the second derivative pre-treatments. After PLS-1 calibration, the prediction accuracy r values were over 0.99, between 1 250-1 667 nm of split spectra.