(NH4)2SO4 on PCR amplification with Tce DNA polymerase.
Amplification of a 2 kb k DNA fragment was performed in
50 ll containing 50 mM Tris/HCl (pH 7.8), 5 mM MgCl2, and
0.01% Triton X-100 with the indicated concentrations of
(NH4)2SO4. d Effect of MgSO4 on PCR amplification with Tce
DNA polymerase. Amplification of a 2 kb k DNA fragment
was performed in 50 ll containing 50 mM Tris/HCl (pH 7.8),
10 mM (NH4)2SO4, and 0.01% Triton X-100 with the indicated
concentrations of MgSO4. Lane M 1 kb DNA ladder (New
England BioLabs)
Biotechnol Lett (2011) 33:339–346 343
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