The means and standard deviations were given as descriptive statistics. All data for normality were tested by using the Kolmogorov–Smirnov test. Per-protocol analysis was used for the comparison of treatment groups. For determining the difference before and after treatment for all groups, non-parametric Wilcoxon test was used. To compare the differences between two groups, the Mann–Whitney U test was used. A level of significance of p < 0.05 was accepted. All analyses were performed using the SPSS for Windows 18.0 software program.