An elicitor has been isolated from Macrophomina phaseolina,
the root rot and leaf blight pathogen of greengram.
Suspension-cultured cells of greengram were established
which responded to the fungal elicitor. When greengram
leaves were inoculated with M. phaseolina two new peroxidases
appeared. Similarly, two new peroxidases could be
detected in suspension-cultured greengram cells when treated
with the fungal elicitor. These peroxidases were purified by
column chromatography and their molecular masses were
27 and 38 kDa. The new peroxidases detected in both leaves
and cultured cells appear to be similar with the same molecular
weights.
Abbreviations: NAA = α-naphthalene acetic acid; BAP =
6-benzylaminopurine; TEMED = N,N,N’,N’-tetramethylethylenediamine
; MS = Murashige and Skoog; PAGE
Polyacrylamide gel electrophoresis; PR = Pathogenesisrelated
; PRPO = Pathogenesis-related peroxidases.