The crude extract of G. changii was screened for DPPH
radical scavenging activity according to the method described
and the results of the screening are shown in Figure 1 as
comparable with known antioxidant.
The methanolic extract tested showed a lower reducing
power activity than BHT and vitamin E. BHT showed the
highest reducing power activity (66.0%) followed by Vitamin
E (40.1%) and the methanolic extract (4.3%) respectively at the
concentration tested (1 mg/ml). We therefore suggest that at
proper concentration of the crude extracts of G. changii may
act as free radicals scavenger and may react with free radicals
to convert them to more stable products and terminate radical
chain reaction [12]. Our result demonstrated that crude extract
of G. changii thereby has moderate free radical scavenging
activity.
The phenolic content of the sample was determined using
Folin-Ciocalteu reagent and was 5.0 mg/g dry weight expressed
as gallic acid equivalents (GAE). Typical phenolics that possess
antioxidant activity have been characterized as phenolic acids
and flavonoids [15]. Phenolic acids have repeatedly been
implicated as natural antioxidants in fruits, vegetables, and
other plants. For example, caffeic acid, ferulic acid, and vanillic
acid are widely distributed in the plant kingdom [16].
From these results it can concluded that the crude extract
of G. changii are promising starting material for the isolation
of compound(s) with antioxidant activities. There is lack of
information available on the chemical composition of G. changii,
which exhibit antioxidant activity. Further phytochemical work
need to be done on these extracts including fractionation to
isolate active constituent and subsequent pharmacological
evaluation.
The crude extract of G. changii was screened for DPPHradical scavenging activity according to the method describedand the results of the screening are shown in Figure 1 ascomparable with known antioxidant.The methanolic extract tested showed a lower reducingpower activity than BHT and vitamin E. BHT showed thehighest reducing power activity (66.0%) followed by VitaminE (40.1%) and the methanolic extract (4.3%) respectively at theconcentration tested (1 mg/ml). We therefore suggest that atproper concentration of the crude extracts of G. changii mayact as free radicals scavenger and may react with free radicalsto convert them to more stable products and terminate radicalchain reaction [12]. Our result demonstrated that crude extractof G. changii thereby has moderate free radical scavengingactivity.The phenolic content of the sample was determined usingFolin-Ciocalteu reagent and was 5.0 mg/g dry weight expressedas gallic acid equivalents (GAE). Typical phenolics that possessantioxidant activity have been characterized as phenolic acidsand flavonoids [15]. Phenolic acids have repeatedly beenimplicated as natural antioxidants in fruits, vegetables, andother plants. For example, caffeic acid, ferulic acid, and vanillicacid are widely distributed in the plant kingdom [16].From these results it can concluded that the crude extractof G. changii are promising starting material for the isolationof compound(s) with antioxidant activities. There is lack ofinformation available on the chemical composition of G. changii,which exhibit antioxidant activity. Further phytochemical workneed to be done on these extracts including fractionation toisolate active constituent and subsequent pharmacologicalevaluation.
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