For measuring emulsification index, hydrocarbons or oils were added to aqueous phase containing the biosurfactant
(0.1%, w/v), in a ratio of 3:2, v/v, followed by vigorous agitation on a cyclo-mixer for 2 min. The oil, emulsion and aqueous layers were measured at every 24 h interval up to 240 h. The emulsification index was noted with respect to time (Cooper & Goldenberg, 1987) and is represented accordingly, i.e. the emulsification indices after 24 h, 48 h, 72 h, etc.
For measuring emulsification index, hydrocarbons or oils were added to aqueous phase containing the biosurfactant (0.1%, w/v), in a ratio of 3:2, v/v, followed by vigorous agitation on a cyclo-mixer for 2 min. The oil, emulsion and aqueous layers were measured at every 24 h interval up to 240 h. The emulsification index was noted with respect to time (Cooper & Goldenberg, 1987) and is represented accordingly, i.e. the emulsification indices after 24 h, 48 h, 72 h, etc.
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