D. melanogaster were reared on media supplemented with
[1-13C]-palmitic acid to investigate if heavy-labeled precursor
feeding is feasible in this model system, as this would aid in future
studies of fatty acid amide metabolic flow. After a 5-day exposure
to [1-13C]-palmitic acid, the lipids were extracted from D. melanogaster
heads to analyze for fatty acid amides that contain the 13CN-palmitoyl
chain as well as the other unlabeled metabolites.