Conclusion
Among four types of pesticides, omethoate, triadimefon and
cyhalothrin did not inhibit but delayed the alcoholic fermentation
at their proposed spraying concentration of 0.21, 0.10 and 0.10 g L1,
respectively. Whereas chlorothalonil concentration above
0.03 g L1 showed significantly negative influence on both S. cerevisiae
growth and alcoholic fermentation, and the fermentation
efficiency would not be interfered with when the chlorothalonil
concentration was lower than 0.01 g L1. It was confirmed that
chlorothalonil hydrolytic dehalogenase (Chd) expressed from recombinant
B. subtilis WB800 could eliminate the inhibition of
0.1 g L1 chlorothalonil substantially when used to pretreat the
chlorothalonil contaminated broth by a Chd amount of 7.25 mU L1
slurry for 60 min. The results of our study suggest a potential
approach to solve the pesticides inhibition in fermentation
industry.