On the contrary, this fragment was not amplified when DNA from S. aureus ATCC 29213, S. agalactiae ATCC 13286 or S. bovis ATCC9809 was used as negative control (Fig. 1;lane 6, and results not shown).
Two PCR products of 112 bp and 732 bp from amplification of the tuf gene and the vanA gene, respectively, were observed when DNA of E. faecium BM4147 (reference strain for vanA) was used (lane 2).