The objective of this study was to determine whether the larvae of Donax trunculus Linnaeus (1758; Mollusca: Bivalvia) could be cultured. This paper reports the results of two experiments to examine larval culture conditions. In the first, two temperatures (17 °C and 20 °C) and the effect of antibiotics were tested. The second experiment was carried out to study the effect of seven diets of different microalgae combinations: Isochrysis aff. Galbana (clone T-ISO), Chaetoceros gracilis (Schutt), Tetraselmis suecica (Kylin) Butcher and Rhodomonas baltica (Karsten) on growth and survival. Best temperature for culture was 20 °C and at this temperature survival was improved using an antibiotic (chloramphenicol 2.5 p.p.m.). Growth was greater with multispecies diets including C. gracilis. T-ISO was a relatively poor food when used as the sole component of the diet.