HeLa T4+, a human cervical epithelial carcinoma cell line that stably expressed CD4
and endogenously expressed CXCR417 and HEK293T, a human embryonic kidney cell line18
(a generous gift from Dr. Thaned Kangsamaksin, Department of Biochemistry, Faculty of
Science, Mahidol University), were maintained in Dulbecco’s modified Eagle’s medium
(DMEM, Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco)
and 1X penicillin/streptomycin (Gibco) at 37C in 5% CO2.