The volume of inoculum plays an important role in the
fermentation of enzymes Lin et al. (1998). In our study,
1% inoculum induced the maximum amylase production
(Table 1). As the inoculum level was further increased, the
production of enzyme was gradually decreased. It may be
due to the fact that at high concentration of inoculum
level, the bacteria grow rapidly and the nutrients present
in the media were insufficient to overcome the growth of
bacteria. Thus, the production of amylase was affected at
higher concentration of inoculum. Our findings are in a
good agreement with Riaz et al. (2003).
Natural sources could serve as economical and