Adult oviposition preference is the key to host selection of leafrollers.
Both E. tetragonana and E. ustulanan larvae are leaf-rollers,
living in the rolls until pupation. Individuals have limited opportunity
to transfer from one plant to another so instead of larvalchoice
tests adult oviposition choice tests were conducted using
plant species that supported larval development in the larval
non-choice tests.
A total of 14 species were tested for E. tetragonana. Since most
of the test species are bushes and hard to manipulate, tests wereconducted on cut shoots (with leaves) that were placed in fine net
cages (1 1 1 m3
) in the laboratory. Plastic bottles with shoots,
about 10 cm in length, were arranged randomly in the net cages.
Due to space limitations, there were nine plant species in Group
1 and six in Group 2, both groups included the control (R. ellipticus)
(see Tables 3 and 4). Five newly mated pairs of adult moths were
released in each cage. Cotton wool with water and honey was provided
as a food source within the cages. All the tests were replicated
six times. After five days, all of the shoots were collected
and checked for eggs under stereoscopic dissecting microscope.
For E. ustulanan, five plant species were tested for adult oviposition.
The experimental design was the same as for E. tetragonana
but all the plants species were in a single group.
We used one-way ANOVA to analyze effects of plant species on
the number of eggs in adult oviposition choice, followed by post
hoc LSD test. The variances of the data were stabilized using
lg(x + 1) transformation in the ANOVAs. The statistical significance
was set at P < 0.05. All data were analyzed by SPSS for Windows
version 16.0.
Adult oviposition preference is the key to host selection of leafrollers.Both E. tetragonana and E. ustulanan larvae are leaf-rollers,living in the rolls until pupation. Individuals have limited opportunityto transfer from one plant to another so instead of larvalchoicetests adult oviposition choice tests were conducted usingplant species that supported larval development in the larvalnon-choice tests.A total of 14 species were tested for E. tetragonana. Since mostof the test species are bushes and hard to manipulate, tests wereconducted on cut shoots (with leaves) that were placed in fine netcages (1 1 1 m3) in the laboratory. Plastic bottles with shoots,about 10 cm in length, were arranged randomly in the net cages.Due to space limitations, there were nine plant species in Group1 and six in Group 2, both groups included the control (R. ellipticus)(see Tables 3 and 4). Five newly mated pairs of adult moths werereleased in each cage. Cotton wool with water and honey was providedas a food source within the cages. All the tests were replicatedsix times. After five days, all of the shoots were collectedand checked for eggs under stereoscopic dissecting microscope.For E. ustulanan, five plant species were tested for adult oviposition.The experimental design was the same as for E. tetragonanabut all the plants species were in a single group.We used one-way ANOVA to analyze effects of plant species onthe number of eggs in adult oviposition choice, followed by post
hoc LSD test. The variances of the data were stabilized using
lg(x + 1) transformation in the ANOVAs. The statistical significance
was set at P < 0.05. All data were analyzed by SPSS for Windows
version 16.0.
การแปล กรุณารอสักครู่..
