3. Results
3.1. Development of callus induction medium
Cotyledon and hypocotyl explants excised from seven days old
in vitro raised seedlings were used for callus induction study. Both
explants formed callus under various hormone treatments.
Hypocotyl explants showed quick response within 5–7 days
whereas cotyledon showed slow response in callus induction
under all hormonal combinations tested. The quality of the callus
was considered to be good on the basis of size, color and texture