Method
In to a series of 25 ml standard flaks containing
Aciclovir drug solution ranging from 0.5-2.5 ml are taken.
To each flask 1ml of 0. 1M sodium hydroxide is added.
These flasks are scanned over the wave length range of of
250-310 nm against reagent blank prepared by taking
various drug solutions (0.5-2.5 ml) in volumetric flasks
containing 1 ml of 0.1M hydrochloric acid solution. From
the absorbance values two wavelengths at 270 and 290 nm
are selected one at positive peak and another at negative
peak, the amplitude is calculated from these values (Patel
Hitesh N et al., 2010; Nanda RK et al., 2009; Gupta SC,
1999; Sandell EB, 1996). The results are presented in
figure 2.
Calibration and amount of drug
The calibration curve is plotted between
amplitude values and amount of drug (concentration of
drug). The calibration curve is found to be linear over a
concentration range of 50 to 250ug/ml of Aciclovir. The
amount of Aciclovir present in the sample is estimated
from the calibration graph. The results are presented in
figure 3.