The bimetallic Pt0.55Co0.45/C and Pt0.7Co0.3/C nanocatalysts wereprepared on the composite surface of carbon by using chemi-cal reduction method. The obtained PtxCo1−xnanoparticles werewell dispersed and exhibited the features of alloy. Electrochem-ical observation showed that the Pt0.7Co0.3/C modified electrodehad large active surface areas and high electrocatalytic activityfor the oxidation of glucose in PBS (pH 7.0) solutions. When itwas used as a glucose sensor, the linear range, sensitivity, repro-ducibility, selectivity and long-term stability were acceptable. Thus,the novel Pt0.7Co0.3/C sensor has potential application in glucosedetection