2.4. Water holding capacity
Approximately 1 g of banana flour was weighed into
pre-weighed 15 mL centrifuged tubes. To each sample, 10 mL of
distilled water was added and mixed for 2 min. Samples were thoroughly
wetted and allowed to stand at room temperature for
30 min after which they were centrifuged at 3000 rpm for
20 min. The resulting supernatant was decanted and the centrifuge
tube containing sediment was weighed. Water holding capacity;
gram of water per gram of protein was calculated thus