Total polyphenols content determination.
1 mL of pomegranate juice was diluted to 10 mL with distilled water
200 μL of diluted juice
mix with 0.5 mL of Foline Ciocalteu reagent, alkalinized with 1 mL of 20 g/100 g of Na2CO3 then adjust the solution volume to 10 ml by distilled water
Let the mixture stand at room temperature, for 60 min
the absorbance was measured by a UV/vis spectrophotometer at 760 nm.
Total phenolics were
calculated against a calibration curve obtained with gallic acid and
results were reported as gallic acid equivalents (mg/L).