Two hours after the venom injection,animals were killed
by ether anesthesia,and the skin covering the abdomen was
removed,stretched,and dried at room temperature for 72h.The
skin was fixed to a lucite base plate,and the entire area at the
injection site and the surrounding area were transilluminated using
an incandescent light.Light transmitted over an area of 109 mm2
was read,and light transmission or the absorbance was normalized