3.2. Real-time PCR analysis
Standard curves of the optimized assays using SYBR Green I were
created. No primer dimers with lower T
values were observed. When
data were compared to HS groups, a significantly greater change in
intestinal bacterial composition was observed in the IBD group (Fig. 1,
Table 3). A statistically significant decrease in the numbers of
Bacteroides–Porphyromonas–Prevotella was observed in both the A-
m