S. cerevisiae haploid strain ZS were derived from industrial strains. The engineered strains in this study were engineered from the wild strain ZS. All strains unless otherwise noted were grown on YPD medium, which contains (m/v) 2% peptone, 1% yeast extract, and 2% glucose.
In other reserach the intracellular trehalose content of all the strains rapidly increased with time, peaking at 20 h under regular fermentation