The extract was filtered through cheese cloth and then through 0.5 mm sieve. The resulting precipitate was dried in oven and then powdered using electric grinder to get lupin flour, whereas, the homogenized filtrate was considered as lupin milk. The milk was thermally treated at 100 °C for 20 min to inactivate the lipoxygenase, and then cooled to 4 °C. The milk stored at 4 °C for further use.