Conclusion
In the present research, 18 CcHsf genes were identified from the
genome database of C. clementina. Based on structural characteristics
of the proteins and the comparison with homologs from other species,
the CcHsfs were grouped into three different clades. MEME motif analysis
highlighted the conserved domains similar to Hsf protein structures
in other species. Temporal expression studies identified 14 CrHsf genes
as potential candidates for a role in fruit development and ripening,
while seven CrHsf genes were expressed in response to hot air stress.
CrHsfB2a and CrHsfB5 play roles in both developmentally-related and
hot air-triggered citrate degradation processes, but the mechanism of
regulation needs further research.