Cell walls thickness of about 5 to 20 nm
functions as natural sieves which transports small
particles passes through large pores to enter in the
protoplasm. Eventually, same authors had also
admitted that new and large pores were created for
passaging of larger particles 1, 2. Breakage of cell wall
and vacuoles in root cells of test species described the
toxicity of particles. Depositions of silver
nanoparticles inside the root cell by smaller particles
have also been reported in our study (Figure 3A).
Different particle sizes inside was observed under
TEM. Presence of variable size and compositions was
also reported in intracellular portions of various plants
species 14. One of the pathways was also reported
where particle size of 20 nm silver nanoparticles may
be transported inside the cells through plasmadesmata
Particles must be entered through cell wall and
plasma membrane of root cells. Xylem is one of the
main passages of uptake and transportations to shoot
and leaves of plant. Pores size of cell wall was in the
range of 3-8 nm, smaller than engineered
nanoparticles16. Damaged in intracellular level may be
due to surface area or size of particles interacts with
plant roots. However more study is required to know
the uptake mechanisms and toxicity of particles in
intracellular damages.