2.2.1. Determination of <5 kDa peptide fraction concentrationThe <5 kDa peptide fraction was separated from other rice branprotein hydrolysates using ultrafiltration and quantified using anHPLC procedure by Kannan, Hettiarachchy, Lay, and Liyanage(2010). The standard curve for the hydrolysates was determinedusing solutions of peptide fraction in deionized water with theeffective concentration as the median. The standards were injected(1000 mL) into a C18 affinity chromatographic column attached toan HPLC system with a flow rate of 2 mL/min using two solvents.
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