Fig. 3. Immunoblotting showing the reactivity of anti-rFgCatB2 sera to native cathepsin B2 of various stages of F. gigantica. The blots were subsequently probed with anti-rFgCatB2 as the primary antibody, and goat anti-mouse IgG conjugated to HRP as the secondary antibody. Lane 1: rFgCatB2; lane 2: WB of metacercariae; lane 3: WB of newly excysted juvenile; lane 4: WB of 2 week-old juvenile; lane 5: WB of 3 week-old juvenile; lane 6: WB of 4 week-old juvenile; lane 7: adult WB; lane 8: adult TA antigens; Lane 9: adult ES. The molecular weight markers are shown on the left side.