(NPBs)
and
nuclear
buds
(NBUDs),
which
may
provide
a
measure
of
genome
damage
and/or
chromosomal
instability.
The
nuclear
division
index
(NDI),
apoptotic
cell
rate
(ACR)
and
necrotic
cell
rate
(NCR)
in
1000
cells
per
sample
were
scored
to
detect
the
cytostasis
and
cytotoxicity.
2.6.
MDA
assay
Lipid
peroxidation
was
evaluated
by
determining
the
rate
of
production
of
thiobarbituric
acid
(TBA)
reactive
components,
which
was
expressed
as
malon-
dialdehyde
(MDA)
equivalents
[30].
MDA
level
in
blood
plasma
(0.2
ml)
of
each
subject
was
measured
using
reagent
kits
(Jiancheng
Bioengineering
Co.
Ltd.,
Nanjing,
China)
according
to
the
manufacturer’s
instruction.
Absorbance
was
determined
at
a
wavelength
of
532
nm
with
a
microplate
spectrophotometer
(Multscan,
Thermo,
USA).
(NPBs) and nuclear buds (NBUDs), which may provide a measure of genome damage and/or chromosomal instability. The nuclear division index (NDI), apoptotic cell rate (ACR) and necrotic cell rate (NCR) in 1000 cells per sample were scored to detect the cytostasis and cytotoxicity. 2.6. MDA assay Lipid peroxidation was evaluated by determining the rate of production of thiobarbituric acid (TBA) reactive components, which was expressed as malon- dialdehyde (MDA) equivalents [30]. MDA level in blood plasma (0.2 ml) of each subject was measured using reagent kits (Jiancheng Bioengineering Co. Ltd., Nanjing, China) according to the manufacturer’s instruction. Absorbance was determined at a wavelength of 532 nm with a microplate spectrophotometer (Multscan, Thermo, USA).
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