To evaluate the influence of alcohol concentration, a series of
experiments was performed at an acid concentration of 0.2 M
and alcohol concentrations ranging from 0.05 M to 0.8 M, resulting
in molar ratios to alcohol to acid (R) of 0.25–4. The data, displayed
in Fig. 2B, showed a significant change in ester yield with the
change in alcohol concentration. The ester yield was 98.3% at the
optimal alcohol concentration of 0.1 M, and decreased sharply to
77.6% at an alcohol concentration of 0.8 M. This phenomenon
was previously reported for other enzymes, such as the cutinase
from F. solani pisi and the whole-cell lipase from Rhizopus chinensis