Nitrocellulose is not a suitable medium for electrophoretic transfer of nucleic acids, as high concentration of salt are required for efficient binding. Molecule น้อกว่า500 are not bound at all, even at high salt. Low resistance results when an electric current is passed through a solution of high salt. This causes potentially damaging high currents even at very low voltages. Since V/cm is the eluting force, inefficient transfer occurs under condition required for proper binding.