Using enrichment in CGY at 50°C with subsequent differentiation of
isolates by FT-IR and confirmation by PCR analysis of the cytK-1 gene,
thermotolerant B. cytotoxicus was isolated and identified with high
specificity. Growth of B. cereus or Bacillus thuringiensis was successfully
suppressed under the chosen conditions. Five isolates that were not
unambiguously identified as B. cytotoxicus by FT-IR analysis were
confirmed by sequencing of their partial gene for 16S rRNA, which
resulted in sequences that showed highest similarities to known 16S
rDNA sequences of B. cytotoxicus.