PCR using the VPI primers for the purified segment of
GS03032 (V. alginolyticus) and for ZJ04124 (V. alginolyticus)
and using tlh primers for VPL4-90 (V. parahaemolyticus)
gave positive results in the second round of semi-nested
PCR, whereas for ZJ03060 (V. alginolyticus) using the tlh
primers we gained no amplification product. The four
specific-size fragments derived from the first round amplicon,
were sequenced and put into Genbank. The accession