The thermal stability profile reported in Fig. 4b showed that the purified enzyme is highly stable at temperatures below 40 C,
retaining 100% of its activity after 60 min of incubation at 30 and 40 C. At 50 C, the enzyme retained about 65% of its initial activity after 60 min of incubation (p < 0.05). However, the enzyme was inactivated at higher temperatures. The thermal stability profile of S. basilisca trypsin was similar to that of grey triggerfish