Inhibition of pathogens was initially observed using spot
on lawn assay. In this method pure culture of pathogen
was used to make a lawn on agar plate it was allowed to
dry for five to ten minutes then a drop of peptide solution
collected from different fermentation conditions was kept
over this lawn at equal distance. These plates were
incubated for 24-48hr at 370C and plate was observed for
inhibition of pathogens