Malaria remains a major cause of morbidity and mortality worldwide. Flow cytometry-based assays
that take advantage of fluorescent protein (FP)-expressing malaria parasites have proven to be valuable tools for
quantification and sorting of specific subpopulations of parasite-infected red blood cells. However, identification of
rare subpopulations of parasites using green fluorescent protein (GFP) labelling is complicated by autofluorescence
(AF) of red blood cells and low signal from transgenic parasites. It has been suggested that cell sorting yield could
be improved by using filters that precisely match the emission spectrum of GFP.