Polymerase chain reaction (PCR) with specific primers was used to amplify the ITS-rDNA regions of potatocyst nematodes(PCN) in soil samples from Indonesia. Amplified DNA fragments showed that a single species, Globoderarostochiensis,withfragmentsize238bpoccurred in East Java, and West Java, while mixed populations of G. rostochiensis and G. pallida with fragment size 391 bp were only found in Central Java