was flushed under a flow of nitrogen and sealed. The sealed ampoules were heated in a water bath at 95°C for 45 min and cooled. After breaking the ampoules, 3 ml of 5% Na2CO3 (anhydrous) solution was added and vortexed. The fatty acid methyl ester was extracted three times with 3 ml of petroleum ether, dried with nitrogen, dissolved in 100 μl of petroleum ether, and 1 μl volumes were injected and analyzed by gas chromatography using a model DS 6200 apparatus (Donam, Korea). Fatty acids were identified by matching their retention times with those of their relative standards (polyunsaturated fatty acid (PUFA)-2; Supelco, USA) and the Food Composition Table (NRLSI, 2002).