3.2. Assessment of the in vitro antioxidant activities of FPP
The antioxidant profile of FPP was established using 7 independent
assay methods using a concentration range varying between 1
and 200 mg/ml. A ranking order indicating the concentration of
FPP required to attain an antioxidant activity of 50% (AA50) for each
assay was established as follows (Table 1): hydroxyl scavenging
(4.13 ± 0.76 mg/ml) > ABTS scavenging (14.56 ± 1.65 mg/ml) >
DPPH scavenging (55.69 ± 6.46 mg/ml) > inhibition of AAPHinduced
lipid peroxidation (68.06 ± 22.79 mg/ml) > superoxide
scavenging (88.70 ± 22.48 mg/ml) > nitric oxide scavenging
(116.55 ± 40.59 mg/ml) > inhibition of iron (III)/ascorbate-induced
lipid peroxidation. The activity of FPP was observed to be dose
dependant in all assays (data not shown).