2.5. Determination of the amount of viable cells in co-cultures by
inoculation onto dense nutrient media (Koch method)
A number of successively diluted suspensions of microbial
co-cultures (degree of dilution, 10−6) were prepared to produce
separate colonies. Suspensions were inoculated by the surface
method from three last dilutions (4 parallel inoculations). Colonies
were counted after 7 days of incubation.