For shoot induction and proliferation, MS media containing different concentrations and combinations of auxins and cytokinins
were used as mentioned in Tables 1and 2. For rooting, MS medium was supplemented with different concentrations of NAA. All the cultures were maintained under light intensity of 2500 to 3000 lux having temperature of 22±2.0°C and photoperiod was 16 h with 8 h dark period in every 24 h cycle. Sub-culturing was carried out after every 4 weeks interval.